Methods:

First we genetically engineered the genome of yeast by knocking out the  TOS3 gene by homologous recombination. Next, we ran a PCR to see if our transformation worked. Once we saw that our transformation worked we moved on to make the media for the experiment. We made four different media, a solid fermentable carbon source (YPD), a solid non-fermentable carbon source (YPE), a liquid YPD and a liquid YPE.  For each solid media both types of yeast strains (wild type and knockout ) were cultured on six different plates (three wild type and three knockout). For both liquid media the wild type and knock out strains were mixed together. All cultures were incubated at 30 degrees Celsius for five days. 

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Measurements:

We measured the area of the yeast cultures on the solid media and compared the two strains. The liquid cultures were measured using the FACS machine to measure the competitive ability of each strain**.

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** The liquid cultures could not be measured because the FACS machine was shut down due to hurricane Matthew.