Yeast Culture/Gel electrophoresis- 09/07/16
Method for Yeast Culture
We had to culture yeast in three 1.5 mL tubes of 10 μL nutrient broth and two agar culture dishes.
1) we inserted 20μL of yeast (taken from master source) via micropipette into each 1.5mL tube
2) 30 mL of yeast was micropipetted into the first dish
3) we had to pipette 20μL of yeast from one source into the second dish then added 10μL from another source
Observations
Observations
While we transferred the yeast onto the solid agar growth medium we noticed that the micropipetter we were using was not accurate. We realized this when the pipetter wasn't releasing all of the sample out of the tip. An inoculating loop is usually used with culturing, however we did not have one so we used the wider end of the pipetting tip.
Gel Electrophoresis
We used gel electrophoresis to see if our PCR worked effectively. We found out that our DNA needed to be 1816 base pairs long, and used PCR to see if the number of base pairs from our samples matched that number.
Our results from gel electrophoresis. This shows that our PCR was succesful.
How to Avoid Contaminarion
It is very easy to contaminate a specimen. Different microbes in the air can end up in your sample if your workspace isn't sterile. To avoid contamination we
1) Wore gloves
2) Wiped our workspace down with 95% ethanol
3) Did our pipetting next to ethanol burners
a) ethanol burners kill surrounding microbes
4) Used sterile pipette tips
Solid Agar Video
Solid Agar Video
Liquid Agar Video
